leky-latky/enzymy/proteazy-rostlinne

Bromelain

• Key enzyme found in pineapple (Ananas comosus (L.) Merr.)
• Proteolytic substance with multiple beneficial
• Anti-inflammatory, immunomodulatory, antioxidant and anticarcinogenic,
• Stem bromelain is very stable when frozen at -20°C
• Complete loss activity occurs when boiled at 100°C for 10’
• Optimum activity has been found at 30°C and neutrality for the glycosylated form.
• Both heating (40-60°C) and acidification (pH 3-4) led to significant inactivation

Plant Proteases

• Time-consuming process
• Papain
• Bromelain
• Keratinases
• Ficin

Bromelain

• From the stem and juice of pineapples
• Major supplier of the enzyme is Great Food Biochem., Bangkok, Thailand
• Characterized as a cysteine protease
• Active from pH 5 to 9
• Inactivation temperature is 70°C
• Lower than that of papain [11]

Keratinases

• Botanical groups of plants produce proteases which degrade hair
• Digestion of hair and wool
• Important for the production of essential amino acids such as lysine
• For the prevention of clogging of wastewater systems [11]

Papain

• Traditional plant protease
• Extracted from the latex of Carica papaya fruits
• Grown in subtropical areas of west and central Africa and India
• Proteinase and peptidase isozymes
• Performance of the enzyme depends on the
• Plant source
• The climatic conditions for growth
• Methods used for its extraction
• Methods of purification
• Enzyme is active between pH 5 and 9
• Stable up to 80 or 90°C in the presence of substrates
• Extensively used in industry
• Preparation of highly soluble and flavored protein hydrolysates [11]