leky-latky/glutaminaza/popis-druhy

Genetika

GLS gene

• Encodes two isoforms
• By a mechanism of alternative splicing [1]
• GLS isoforms require a polyvalent anion to be active

Kidney-type glutaminase (KGA)

• A long KGA transcript
• First isolated from brain
• Contains exons 1-14 and 16-19 of the GLS gene (Nagase et al., 1998; Holcomb et al., 2000; Porter et al., 2002) [1]

Glutaminase C (GAC)

• Shorter GAC transcript
• Formed by the first 15 exons
• Originally described in human colon cancer cells (Elgadi et al., 1999) [1]

A third transcript (GAM)

• Found in heart and skeletal muscle
• Much shorter protein
• no measurable catalytic activity (Elgadi et al., 1999) [1]