potraviny/clitoria-ternatea/vyuziti-zemedelsko-prumyslove

Butelase 1

• Enzymes that catalyze efficient macrocyclization or site-specific ligation of peptides and proteins
• Can enable tools for drug design and protein engineering
• Peptide ligase
• High-efficiency cyclization and ligation of peptides and proteins
• Ranging in size from 10 to >200 residues
• Fastest known ligase
• Found in pods of Clitoria ternatea
• Simple C-terminal-specific recognition motif that requires:
• Asn/Asp (Asx) at the P1 position [6]
• Dipeptide His-Val at the P1' and P2' positions
• Substrates for butelase-mediated ligation can be
• Prepared by standard Fmoc (9-fluorenylmethyloxycarbonyl) chemistry
• Recombinant expression with the minimal addition of this tripeptide Asn-His-Val motif at the C terminus [6]
• Butelase 1 achieves cyclizations that are 20,000 times faster than those of sortase A, a commonly used enzyme for backbone cyclization
• Butelase is traceless
• Can be used for the total synthesis of naturally occurring peptides and proteins [6]
• Useful for intermolecular ligations and synthesis of peptide or protein thioesters
• Versatile activated intermediates necessary for and compatible with many chemical ligation methods [6]

Ornamental plant

Revegetation species

• In coal mines in Australia
• Requiring little care when cultivated

Improve soil quality

• As legume, its roots form a symbiotic association with soil bacteria known as rhizobia
• Transform atmospheric N2 into a plant-usable form - nitrogen fixing
• Used to improve soil quality through the decomposition of nitrogen rich plant material [1]

Vychytávání formaldehydu ze vzduchu

• Phytoremediation could be a cost-effective, environmentally friendly approach for the treatment of indoor air
• Plant growth-promoting (PGP) endophytic Bacillus cereus ERBP (endophyte root blue pea), isolated from the root of Clitoria ternatea
• Can colonize and stabilize within Zamioculcas zamiifolia and Euphorbia milii
• As non-native hosts without causing any disease or stress symptoms [7]
• Of B. cereus ERBP
• Impact on the natural shoot endophytic community
• Airborne formaldehyde removal capability of non-native hosts [7]
• Non-native Z. zamiifolia was effectively inoculated with B. cereus ERBP through soil
• The most efficient method of endophyte inoculation
• B. cereus ERBP
• Conferred full protection to its non-native host against formaldehyde phytotoxicity
• Enhanced airborne formaldehyde removal of Z. zamiifolia [7]
• Non-inoculated plants suffered from formaldehyde phytotoxicity
• Their natural shoot endophytic community was detrimentally affected by formaldehyde
• B. cereus ERBP inoculation into non-native E. milii deteriorated airborne formaldehyde removal of the non-native host
• Compared to a non-inoculated one
• B. cereus ERBP interfered with natural shoot endophytic community of E. milii
• Caused stress symptoms and stimulated ethylene biosynthesis [7]

Změna barvy anthocyanin-rich extract from butterfly pea petal

• A spectroscopic study - colour degradation mechanism:
• Four different solvent systems:
• Buffer solution pH 7 (AQ7)
• Colour degradation in AQ7 was through the deacylation [5]
• +methanol (ME7)
• Colour degradation in ET7 was due to deacylation and hydrophobic interaction unfolding [5]
• +ethanol (ET7)
• Colour degradation in ME7 was due to deacylation and hydrophobic interaction unfolding [5]
• +acetone (AC7)
• Colour degradation in AC7 was proposed to occur through hydrophobic interaction unfolding [5]
• Colour and anthocyanin degradation from the biggest was:
• AQ7 > ME7 > ET7 > AC7
• Longest hypsochromic shift was:
• AQ7 > ME7 > ET7
• AC7 the shift was absent
• Volume of package headspace provoked colour stability
• Colour degradation in ME7 and ET7 was due to deacylation and hydrophobic interaction unfolding [5]