Cold plasma
Angiogeneze
- Plasma-inducing angiogenesis have been reported by many groups
- FE-DBD treatment could induce angiogenesis
- By FGF-2 release regulated by plasma-produced ROS
- Healing process was improved
- Quantity of neovascular vessels was increased after plasma treatment
- Vascular wound area decease of plasma treated wound was more significant [1]
Cold plasma
- Umí působit nekrozu buněk - dá se cca dobře zacílit
- Proto je zvažováno použití v ter. nádorů
- Více civlivé buňky byly el. vodivější
- books.google.cz/books?id=bzxZkEcLo1kC&pg=PA355&lpg=PA355&dq=cold+plasma+effects&source=bl&ots=W-RNDYpXcA&sig=ACfU3U0VYwdJc8tzs-5BMHC1HqnBuqVYkQ&hl=en&sa=X&ved=2ahUKEwjZxefN8dPkAhUai1wKHeKJBxc4ChDoATAoegQIMRAB#v=onepage&q=cold%20plasma%20effects&f=false
Literatura:
[1] www.intechopen.com/books/plasma-medicine-concepts-and-clinical-applications/cold-atmospheric-pressure-plasmas-caps-for-skin-wound-healingTkáně
- It is generally accepted that low dose of plasma treatment could stimulate cell viability and enhance proliferation, differentiation, and migration, while high dose induces cell apoptosis/necrosis
- It has been found that the resistance against plasma treatment is different between cancer cells and normal cells, which makes plasma selectively killing cancer cells while bring less damage to normal cells and become a potential and powerful tool against cancers
- Increased b1-integrin expression
- Reduced E-cadherin and EGFR expression of HaCaT-keratinocytes after 30 s treatment
- Intracellular level of ROS increased after SMD treatment without dependence on the treatment time or different treatment regimens
- KINPen 09 plasma treatments could also induce oxidative stress in human keratinocytes
- Induce cell reactions of stress-sensing
- Stimulating doses of plasma treatment may protect epithelial skin cells in wound healing
- By promoting proliferation and differentiation
- Hormesis-like processes
- Short-term plasma exposure could enhance keratinocyte proliferation [2]
Cold gas plasma production
Exposing atmospheric gases to high energy in the form of
- Electrical discharges
- Microwaves at atmospheric pressure
- Ionizes the gas
- Generates reactive components like
- Reactive oxygen species (ROS)
- Reactive nitrogen species (RNS)
- UV-light
- Ions and electrons [1]
- Depending on the utilized gas also produced
- Nitrous oxide (N2O)
- Carbon dioxide (CO2) [1]
In nature
- Found in large quantities in the Earth’s ionosphere [1]
Cold solution plasma or liquid-phase plasma
- Generated by exposing solutions (hexane (C6H14), sodium acetate (CH3COONa), or sodium sulfate (Na2SO4) solutions for example
- To high energy via microwaves
- By channeling air-based cold plasma through the solution, at atmospheric pressure
Plasma activated water (PAW)
- Generated by electrical discharge
- Solution plasma also remains at room temperature
In nature, PAW in the form of rain is generated during lightning [1]
